Zhang Lin took out the cylinder from the liquid nitrogen tank and took out a cryopreservation tube from the cylinder. He then carefully put the cylinder back because there were many cryopreservation tubes of other cells in the cylinder. After Zhang Lin sealed the lid of the liquid nitrogen tank, he began a series of patient and meticulous operations.
He first put the cryopreservation tube in a water bath and quickly thawed it with 37 ℃ hot water. Then he changed the centrifuge tube and centrifuged it for five minutes. Then he sat down on the super-clean workbench and put the mouth of the centrifuge tube under an alcohol lamp to disinfect it. He discarded the waste liquid and used a pipette gun to add fresh culture fluid and mixed it repeatedly.
Gu Jun watched carefully. If he had any questions, he would ask Senior Zhang and he learned a lot.
For example, the speed control of the centrifuge and the suction and discharge control of the pipette gun. If these recovery operations were sloppy, it would affect the survival and proliferation rate of the cells.
Finally, Zhang Lin injected the cell suspension into a petri dish, added the right amount of culture fluid, and put it into a 37 ℃, 5% CO2 cell incubator.
"Wait, it will take at least two days." Zhang Lin took off his mask and pushed up his glasses. "I suggest you rest for a week so that the tumor formation rate can be guaranteed."
"I'll just try a batch for two days first." Gu Jun shrugged. There was no other way. There was not enough time. The system showed that his condition would worsen after 30 days. If it was true, it might be difficult for him to even get out of bed and walk. He said, "Senior Brother, want to go out for a meal tonight? It's my treat. "
"No thanks." Zhang Lin waved his hand. "I still have to feed the mice tonight. It's my treat."
…
For the next two days, Gu Jun spent his time on campus. In addition to catching up with friends, he read books and prepared for the make-up exam. It would be quite troublesome if he didn't die but had to repeat a year. Secondly, he went to the laboratory building every day to take care of the cells and change the culture fluid.
On the morning of the third day, Zhang Lin personally came to take a look and said that the cells had grown enough. He helped with the passage culture and the preparation of the cell suspension.
Gu Jun went to the school's Animal Laboratory Center. It was actually a large breeding base located a few hundred meters away from the school park. It was very lonely, probably because it was filled with the stench of various animals.
He bought five naked mice, which cost more than 100 yuan each. It was quite expensive. Fortunately, he still had 20,000 yuan in his savings, but it wouldn't last long.
Gu Jun really regretted donating the money so early … He was still alive, but the money was gone.
Cough. Each of these naked mice was the size of two or three fingers put together. They had no hair and were stored in a sterile box. Once they came into contact with the outside world, they would easily get infected and die of illness. Therefore, the process of raising and conducting experiments had to be carried out in a barrier environment.
Eastern University's barrier environment laboratory was located in the Animal Research Center. This five-story building with yellow brick walls stood right next to the laboratory building.
Gu Jun and Zhang Lin met in the lobby on the first floor of the research center and booked a small naked mouse room for one hour. It was a small room of only 21 square meters. It was divided into a front room and a back room. The front room had a laboratory table and instruments, while the back room was a breeding room.
Before entering, they and all the items they brought with them had to be sterilized with ultraviolet light. The two of them were fully equipped with special full-body protective clothing, masks, gloves, and foot covers before stepping into the naked mouse room.
Soon, on the laboratory table in the front room, Gu Jun marked five naked mice with a perforation method, and then injected tumor cells subcutaneously on the right side of their buttocks one by one.
Zhang Lin was responsible for assisting next to him. These cells could theoretically form tumors if they were injected into a human body. Now that he saw Tyrant Jun so enthusiastic about the naked mice, he was sure that this little brother did not want to play murder.
After all five naked mice were injected, Gu Jun put them in the cage and then saw if they could form tumors.
"Senior brother, thank you." Gu Jun gave senior brother Zhang a thumbs up. Without senior brother's help, things would not have gone so smoothly.
"The old Chinese medicine doctor you mentioned …" Zhang Lin still had a vigilant look on his face. "Is he really not a liar?"
...
For the next week, Gu Jun continued his previous pace of life, but he went to the naked mouse room every day to look at the cage of naked mice. Their feeding was taken care of by the management of the naked mouse room, and each one cost 3 yuan a day for food and accommodation.
In this week, he only completed three ordinary tasks because he could not do those that required treating patients and saving people. Although the hospital was next to him, he was not a doctor. The tasks he completed were related to medical practice. There were already five boxes of pills in his hand, and the proficiency of the Hands of Dexterity had reached the first level (3000/5000).
On the seventh day, Gu Jun once again walked into the naked mouse room in sterile equipment and observed the naked mice one by one.
None of them died. A soybean-sized tumor had grown under the skin on the right side of No. 1's buttocks. After measuring, it was 0.5 cm in diameter. No. 3 and No. 4 also had obvious protrusions, with diameters of 0.3 cm and 0.5 cm respectively. No. 2 and No. 5 still had no signs of tumor formation.
At this point, he could be sure that out of five naked mice, three had tumors.
Gu Jun set two tumor-forming naked mice as Group A, one tumor-forming naked mouse as Group B, and the two non-tumor-forming naked mice as Group C and Group D. He also went to the animal center to buy 10 healthy naked mice. Five were set as Group E, and the other five as Group F. Group A, Group C, and Group E would all receive the same dose of "foreign medicine" (the name he gave) every day, while Group B, Group D, and Group F would not receive any medicine and would only be used as a control.
That was, tumor-forming naked mice, non-tumor-forming naked mice, and healthy naked mice, each group would receive medicine, and the other group would not receive medicine.
At this time, the experimental table had been divided into six cages, and the naked mice were chirping noisily.
Gu Jun fiddled with the 25 foreign medicine capsules. Since the system said that one box could extend life by five days, and there were five capsules in a box, it should be one capsule a day. This was the dosage for adults, and the average weight of these naked mice was less than 20 grams. If they also received one capsule a day, they would probably be sacrificed immediately.
He poured out all the powder in one capsule and measured the dosage. Then, he used his own weight to calculate the dosage for the naked mice, mixed it into a solution, and diluted it according to the ratio.
Soon, Gu Jun held the intravenous injection filled with medicine in one hand and a healthy naked mouse in the other. He began to inject the medicine into the stomach of the first one.
"Squeak, squeak, squeak." The naked mouse struggled a little, but the poor thing was still given a shot of medicine by him, making its contribution to medicine.
At this moment, Gu Jun's heart was beating a little fast. He stared at the naked mouse in his hand without blinking.
He had never done a toxicity test for the foreign medicine until this moment. If it suddenly died, perhaps this experiment would have lost its meaning. But now, he stared at it for five minutes, followed by ten minutes...
Half an hour passed, and this naked mouse still did not show any abnormalities.
"It seems that the foreign medicine does not have obvious acute toxicity." Gu Jun breathed a sigh of relief. "The chronic toxicity needs to be observed."
Only then did he continue to inject the medicine into the next naked mouse. After spending ten minutes to complete the administration of the other seven naked mice in the three dosing groups, he observed them for another hour. None of them had any abnormal reactions. They could still move, cry, drink water, and eat feed.
"My fellow mice, please live on." Gu Jun grumbled. He pressed his throbbing head. His condition was clearly worsening. "Please."
...
The next day, the third day, Gu Jun continued to come to the naked mouse room every day to work.
The tumors of the naked mice in group B had increased from 0.3 cm to 0.6 cm, but their weight and mental state had not changed. The two naked mice in group A had not changed in size after two days, maintaining the size of the tumors at 0.5 cm.
Gu Jun knew what this might mean. The foreign medicine really had an effect! These capsules could significantly inhibit the growth of tumors.
Moreover, the naked mice that were given the medicine were normal, which also meant that the foreign medicine did not seem to have any adverse reactions and did not cause much damage to the body's functions.
"Maybe this time, there is really hope?" He thought to himself. Although the uneasiness still lingered in his heart, a new hope burned at the same time.
The fourth day, the fifth day... In the blink of an eye, a week had passed.
The tumors of the naked mice in group B had grown to 1.5 cm. The huge tumor formed a sharp contrast with its thin body. It began to lose weight and its spirit became worse. The condition of the two naked mice in group A continued to be stable. All the naked mice that were given the medicine were alive and well, no different from the naked mice in the blank control group.
It had to be said that the results of this experiment were very satisfactory.
The efficacy of the foreign medicine was better than the current top small-molecule tumor targeting drugs on the market.
However, Gu Jun also knew that the adverse reactions of the foreign medicine could not be completely ruled out. This only proved that there were no side effects in a short period of time. After all, this was an animal experiment, which was somewhat different from human clinical experiments.
However, the results of this experiment were already worth the risk.
That afternoon, after Gu Jun wrote his will, he came to the waiting area of the emergency department of Eastern University Hospital and sat down on a rare waiting bench. The surrounding area was crowded with patients and their families. Most of them were crying children and comforting parents. Nurses hurriedly walked around, and care workers pushed rescue beds...
It was noisy. The emergency department broadcast continued to call out numbers, "182, Chen Ziyang. 183, Wang Yuxuan. 184, Huang Zixuan... "
Exhaling, Gu Jun decisively threw a foreign medicine capsule into his mouth, drank a mouthful of mineral water, and swallowed it.
"Come on." With a calm face, he looked at the emergency sign on the wall. "If there are any toxic reactions, come on."
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