Cai Zixuan looked around and said, "The leader of our group is Wang Ruoxiang."
"That's it?" Gu Jun frowned helplessly. He thought it was something serious, "I'm not surprised at all. I'd be surprised if it's not her."
"Yeah, that's Wang Ruoxiang we're talking about." Cai Zixuan sighed emotionally.
Wang Ruoxiang was their class monitor. She was capable, beautiful, and had a good personality. Such a goddess, of course, had pursuers from the teaching building to the dissection building. Tycoon Jun was one of them. Back then, he pursued her vigorously, but in the end, Wang Ruoxiang rejected him in public, "I'm sorry, we're not the same kind of people. I don't like people like you."
Well, a tall, handsome, rich, and an eight-year student was actually "this kind of person"?
At that time, everyone said thank you to Tycoon Jun and the class monitor for making them believe in love again.
"The class monitor is still single." Cai Zixuan obviously didn't know how much of a busybody he looked now.
"Don't look at me like that." Gu Jun laughed at himself, "It's all in the past." Indeed, it was all in the past. Tycoon Jun pursued the class monitor partly because he liked her, and partly because he wanted to win everyone's goddess. In the end, it had nothing to do with those deep things.
"Really?" Cai Zixuan didn't continue to talk about the class monitor. He looked at his watch, "Hey, it's almost 8 o 'clock. I have to go to the laboratory."
"Let's go, I'm also going to the laboratory building." Gu Jun said.
Cai Zixuan suddenly showed a look of understanding. Just now, he said he didn't miss the class monitor, but now he wanted to join in the fun. It's good to be young.
"What?" Gu Jun was really helpless. He wanted to explain, but he didn't know where to start. "Forget it …"
"Tycoon Jun, you don't need to say anything. I understand. I understand."
"You understand my a * s."
The Medical Laboratory Building was located in the northwest of the campus. It was a ten-story white brick building with no special features on the outside. Even the entrance was a rotten cement floor that had been in disrepair for a long time. However, there were laboratories with all kinds of equipment in the building for the teachers and students to use in scientific research.
"This way," After entering the building, Cai Zixuan walked in front and explained the situation to Gu Jun.
The name of the experiment that their team was doing was "Research on Photodynamic Therapy of Tumors with Several Photosensitizers". It was a study that implanted tumors under the skin of mice, and then used different photosensitizers to carry out photodynamic therapy, and then observed the changes in the tumors.
The funding allocated this time was only 30,000 yuan, which was considered a lot for an undergraduate research experiment, but an imported pure Photofrin photosensitizer would cost tens of thousands of yuan. In order to make a few more photosensitizers, Wang Ruoxiang carefully calculated and saved money to set up a domestic imitation Photofrin group, dotipofen group, ALA group, and a blank control group, a total of five groups of mice.
"The research proposal and the opening report were all done by the team leader," Cai Zixuan couldn't help but sigh as he spoke. "She really lives up to her reputation as Wang Ruoxiang."
Although Professor Gu was a mentor, he would only occasionally take time to guide them. For an old man like him, rushing for a high-scoring SCI article was his business. Therefore, the Pioneer Cup still depended on the students themselves. The team leader did the most work, but it was an opportunity that everyone yearned for.
Because if the experiment produced a paper, Wang Ruoxiang would be the first author, and Cai Zixuan and the others could only be ranked behind.
"We are still in the first stage, which is to establish the ascites tumor-bearing mouse model. It has been cultivated for a week, so we can't see it today."
After Cai Zixuan said a lot, he suddenly realized that Gu Jun didn't speak for a long time. He seemed to frown when he heard the word tumor …
"Uh, tycoon Jun … You know, the success rate of modeling is not 100%."
Cai Zixuan scratched the bald spot on his head. He really didn't know if Gu Jun understood what he had just said.
"How can there be a 100% success rate in this world?" Gu Jun lowered his head and covered his face. Friend, I'm not that bad at studying, okay?
"Indeed." Cai Zixuan was touched by this sentence, and his face was full of vicissitudes. "Everything has its laws, it is like a dream, an illusion, a bubble, and a shadow."
Well, Gu Jun didn't understand this …
As they talked, the two took the elevator to the eighth floor. The laboratory used by their group was on this floor.
Walking along the corridor to the door of the laboratory, Cai Zixuan opened the door and walked in first. He saw that all the team members had arrived, and he was the last one.
"Zixuan, we were waiting for you."
"Huh?"
At this time, everyone saw a person walk in. The laboratory suddenly became quiet, and only the squeaking of mice could be heard.
Tycoon Jun …?
Didn't they say he dropped out of school to travel around the world? Didn't they say he died? Didn't they say he …
"Hello everyone." Gu Jun smiled and waved to everyone. He looked at Wang Ruoxiang and said, "Hello, class monitor."
Wang Ruoxiang stood beside an experiment table. She had a head of short, black hair, and a delicate oval face. Although she did not have any makeup on, she was still very beautiful. Her tall figure was covered in a white coat, and she looked very heroic. Wang Ruoxiang saw him and frowned slightly. "Hello, Gu Jun." Her voice was also very pleasant to listen to.
Gu Jun nodded in response. After three months, the class monitor was still so beautiful, but he was no longer the same as before.
"Tycoon Jun took a break from school, but he's back now." Cai Zixuan explained to everyone, and then introduced to Gu Jun: Xu Hai, a classmate from the clinical department, Zhang Haoran from the basic medical department, and He Yuhan from the pharmacy department. A group of five people were all here.
Cai Zixuan asked, "Class monitor, Tycoon Jun wants to take a look with us. Is that okay?"
Wang Ruoxiang silently pursed her lips and said, "Just stand aside and don't cause trouble."
Tycoon Jun. She muttered in her heart, thinking of a few years ago when school started, she had a superficial good impression of him … Ugh. This kind of person, lazy, full of all five poisons, rotten wood that can't be carved, mud that can't be put to the wall … Worse than a mouse! Maybe only being dragged to be cut into pieces for research would contribute to medicine.
"No." Gu Jun stood aside obediently, but he always felt that Wang Ruoxiang's eyes were not friendly … It was probably an illusion.
There was already a row of mouse cages on the laboratory table, and each cage contained a group of white Kunming mice. This was the most commonly used experimental animal. It was very docile and small, 1-1.5 months old, weighing only 18-22g, and less than 10cm in length. It could be used for experiments.
Except for a cage of modeling mice, the rest were all blank mice that Wang Ruoxiang had just bought from the school's experimental animal center. Once the modeling was successful, they could be put to use.
Since the team members were all present, everyone immediately began to work.
Crack! Wang Ruoxiang put on rubber gloves and went to the modeling mouse cage to catch mice. These mice were labeled with a dye method. She caught mouse number 1. Cai Zixuan and a few others were responsible for assisting, recording, videotaping, and so on.
"Day eight of S180 mouse fibrosarcoma implantation." Wang Ruoxiang's expression was focused and serious. Her hand gently pressed the obviously bulging abdomen of mouse number 1. The mouse then let out a painful squeak and struggled, but it was firmly held in her hand.
She said, "Mouse number 1's ascites continue to grow. It is already a frog's abdomen. It is very active and does not need to be killed for the time being."
Hearing what she said, the team members suddenly became a little excited, and they all came over to take a closer look. "So this is the 'frog's abdomen.'"
"As expected of a frog's abdomen. Its abdomen is really big." Cai Zixuan sighed.
Although they were all eight-year students, it was really the first time they were doing this kind of real scientific experiment.
"Oh." Gu Jun also came over to take a look. He still understood what they were doing.
The reason why they first created ascites type tumor-bearing mice was to obtain a large number of highly active S180 tumor cells. One had to know that the original cell line was very expensive. One tube was only enough to inject one mouse, and its activity was very weak. They set up four treatment groups, and they needed to cultivate hundreds of mice.
Therefore, they first created and then expanded. Now that the mice had grown frog's abdomen, they could "collect the ascites."
"Gu Jun." Wang Ruoxiang suddenly turned her head and glanced at him. "Can you move away a little? You're in the way. "
"Sure." Gu Jun shrugged and walked away. You have a good aura. I can't even stand a meter away from you.
Cai Zixuan and the others all pretended not to see it. Wang Ruoxiang continued with the next step.
She used a 5ml syringe to pierce the abdominal cavity of mouse number 1 and successfully extracted a full tube of clear yellow ascites. She then injected it into an Eppendorf conical centrifuge tube and took it to a centrifuge for centrifugation. Once centrifugation started, a large amount of white sediment could be seen at the bottom of the centrifuge tube. That was the tumor cells.
"Mouse number 1 modeling successful." Wang Ruoxiang was relieved and happy. She smiled at the team members and said, "There are a large number of S180 tumor cells in the ascites!"
"Yeah!" "Haha." They were all young people. No one cared about the mice's feelings. They cheered. Cai Zixuan praised, "The first step to success!"
The first step was successful. The next step was to give these cells to the treatment group to create subcutaneous tumors. After the tumors were formed, photodynamic therapy would be carried out.
There were still many things to do. They continued to work. They removed the supernatant from the centrifuge tube, redid the suspension count, and adjusted the cell concentration …
In order to maintain cell activity, all injections had to be completed within an hour. Therefore, they were busy with modeling mice before moving on to the next one.
"Adjust the cell concentration to 1 × 10 ^ 7/ml …"
"Yuhan, you're responsible for extracting 0.2ml of cells per needle."
"Leader, the anesthetic is ready!"
Everyone was in high spirits. No one cared about Tycoon Jun anymore. They even forgot that he was standing on the side watching.
Gu Jun watched quietly. He watched He Yuhan mix the cell suspension, Cai Zixuan anesthetize the healthy mice with sodium pentobarbital, Xu Hai and Zhang Haoran label the mice and remove the hair on their buttocks, and finally, Wang Ruoxiang inject tumor cells subcutaneously into the right buttocks of the mice …
Number 1, number 2, number 3, one after another.
Suddenly, Gu Jun felt a splitting pain in his head. Was it a brainstem tumor?
The pain suddenly intensified. He couldn't help but scream, "Ah!" The scene in front of him became blurry, flickering like the shadow of a nightmare.
He seemed to be unable to breathe. It was as if he was in a broken laboratory. Centrifuges, microscopes, sterilization pots, and other various instruments were lying on the ground. Broken test tubes, messy documents, blurred photo frames, and rotten animal carcasses … Dust turned everything into a gray color. There was no breath of life, only chaos and silence.
The pain in his brain was getting worse, but the scene became clearer. He could see some indescribable instruments.
And the twisted carcasses on the ground … He couldn't tell what kind of animals they were …
He also saw a line of words painted in blood on the wall inside. It was the same mysterious language as on the medicine box, but compared to the standard print, every stroke of the bloody words had an eerie strangeness to them.
In a trance, Gu Jun suddenly seemed to be able to understand the meaning of the bloody words.
He murmured softly.
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