Two months later.
Chen Chen stood quietly in the laboratory, his face a little pale.
In front of him, a little white mouse was constantly swimming in a Morris water maze.
This little white mouse was extremely agile when it swam. Although it looked a little lost, it could always quickly find the right path after a few wrong attempts, and finally found the hidden underwater platform.
No one could tell that these mice still had signs of serious neurological damage two weeks ago.
As Chen Chen watched mouse number five come to the platform, he gently pressed the timer and said with a blank expression, "28 seconds, two seconds faster than yesterday."
Following that, Chen Chen began to fill in the experimental records:
"Six-month old genetically modified model mice were treated with surgical transplantation of cloned neural stem cells. At the same time, they were treated with myelocyte triggering receptor 2 (Trem2) agonist interleukin 4 (IL-4) and induced microglia activation.
"Immunohistochemistry, immunoglobulin blotting, and fluorescent quantitative PCR were used to detect the expression of Trem2 at both the protein and gene levels.
"Water maze experiment results: The model mice showed recovery of cognitive function in the water maze experiment.
"In this model, after IL-4 treatment, the expression of Trem2 increased and the memory and cognitive impairment of the model mice was greatly improved.
"Results: The activation of Trem2 may promote the reduction of autophagy of microglia through an inflammatory response."
Following that, Chen Chen bent down and caught the little white mouse that was resting on the platform. After wiping it dry, he fixed it on the CT machine and fixed the brain to a state where it could not move.
Following that, Chen Chen started the machine and the CT machine began to scan the brain structure of number five.
The new CT was compared with the previous image. On the image, the brain structure of the little white mouse was much fuller than it was last week. There was a huge change compared to two weeks ago.
If the brain of the little white mouse was like a shriveled walnut two weeks ago, then it had become much fuller now, like an inflated dough.
Chen Chen casually organized all the data into the corresponding categories. He looked at the few remaining white mice in the experimental cage that were performing similarly and closed the cage.
After that, Chen Chen turned around and held the head of mouse number five. At the same time, his right hand pinched the root of the mouse's tail and gently pulled.
"Crack!"
There was a crisp crack. Number five in his hand died in a swift and effective cervical dislocation.
Chen Chen placed the mouse on the dissecting table and began dissecting its brain.
More than ten minutes later, the brain of No. 5 was completely separated. It was only at this point that Chen Chen noticed that the brain of this mouse did not recover well.
Because of the death of a large number of neurons caused by Alzheimer's disease, many connective tissue blocks were produced in the dead areas of these nerve cells. These tissue blocks occupied the original space of the brain, so the brain could not return to its previous state.
"What a pity."
Chen Chen shook his head and soaked the brains in a formalin-filled solution.
Over the past three months, Chen Chen had used the cloning method to clone this group of mice as usual. At the same time, he used the compound treatment method on these mouse models that also had Alzheimer's disease.
1. Use the gene overexpression of TOM1 protein to increase the brain's resistance to inflammation and gradually eliminate inflammation.
2. Use the solanezumab antibody developed by Eli Lilly and Company to clear the aβ protein and amyloid deposits in the brain.
Although this drug had failed several clinical trials and was proven to be unable to treat Alzheimer's disease, its ability to clear Aβ was still worthy of recognition.
3. Inject neural stem cells extracted from cloned embryos and use cytokines to activate the G2 resting stem cells that originally existed in the brain to stimulate the brain's self-healing.
There was also the final item, which was to first activate the microglia in the early stages of Alzheimer's disease to actively engulf the abnormally deposited proteins.
However, if Alzheimer's disease had entered the middle or late stage, the opposite method was used, which was to inhibit the activation of microglia until the solanezumab antibody was used to clear most of the aβ deposits before activating the microglia.
This was the method Chen Chen had adopted. Through the treatment of inflammation, neural stem cell transplantation, and the intervention of the brain's own immune system, this compound method could completely suppress Alzheimer's disease in the early and middle stages, preventing it from worsening or even improving.
In reality, it was not that the medical world could not achieve the effect Chen Chen had. The only limitation was still the aspect of cloning. Apart from this limitation, the cost was also a factor that limited this treatment.
After all, the cost of this treatment plan alone was sky-high. If it was commercially used, a course of treatment would cost up to a million US dollars.
As for the steric effect caused by the death of a large number of neurons and the occupation of the brain by connective tissue, Chen Chen was at his wit's end. Perhaps it was a good idea to remove these connective tissues through craniotomy.
As for the NZT-48, due to the lack of time and the lack of any practical progress, Chen Chen stopped the experiment.
Since the NZT-48 experiment had been stopped, Chen Chen also began to call for assistants and assign some of the less important tasks. This was how he had obtained these experimental results so quickly.
Now that the experiment had yielded results, Chen Chen no longer hesitated. He immediately asked his assistants to send in the old chimpanzee that had been prepared beforehand.
The scientific community had long discovered that chimpanzees could also suffer from the same Alzheimer's disease as humans. Therefore, before the mouse experiment was completed, Chen Chen had his assistants make an Alzheimer's disease model of chimpanzees and cloned these chimpanzees.
This way, a lot of time could be saved.
Not only that, Chen Chen also had someone from the team protecting his parents in Mainland China go to Shangdu to secretly extract somatic cells from Professor Wang Xi and bring them back to clone Professor Wang Xi. When Professor Wang Xi came to the research center a few months later, the cloned neural stem cells could immediately be used.
Putting in so much effort was also a form of repayment to Professor Wang Xi.
With this in mind, Chen Chen was just about to proceed to the next step of the experiment when his phone suddenly rang.
Chen Chen frowned. He picked up the phone and saw that it was a call from the screenwriter in charge of the new movie.
Chen Chen left the laboratory to his assistants, walked out of the laboratory, and answered the call.
"It's done? I got it, I'll check it out later. "
It turned out that the screenwriter had once again revised the script according to Chen Chen's request and submitted it to Chen Chen for review.
This was the task that Chen Chen had entrusted to the Edwards family.
When Chen Chen arrived at his own office, Little X had already opened his mailbox and projected the script sent by the other party on the wall.
Chen Chen spent a few minutes going through the script, then nodded reluctantly.
The story of the script was set three years in the future. This was Chen Chen's request. After all, the USB flash drive only accepted movies that were close to the real world. It was safer to set it directly in the real world.
The main plot of the story was about an unknown pharmaceutical company in North America. A researcher by the name of Alexander suddenly discovered a small chemical molecule that could completely suppress Alzheimer's disease.
Not only could this small molecule prevent tau nerve tangles and Aβ deposition, but it could also eliminate inflammation and completely end all symptoms of Alzheimer's.
This drug was the core of the story.
The plot was that an unknown underworld force obtained this information from somewhere. In order to obtain a sample of the drug, they began to kidnap the colleagues of the protagonist. When the protagonist discovered the situation, he quickly took the drug and fled, constantly being hunted down by others.
This script had been passed by Chen Chen several times in the past. In desperation, the screenwriter removed some parts of the movie that did not make sense due to technology and logic. These parts were very dramatic and sold well.
Still, this time, it was more or less in line with Chen Chen's expectations.
It was just a shame that Chen Chen did not expect Hollywood to be so slow. It took two months from writing the script to revising it. There was only one month left, no matter how fast they worked, to produce the movie.
Fortunately, Chen Chen had found a treatment to suppress Alzheimer's disease. As long as it was successful on the chimpanzees, Chen Chen could enter clinical trials.
Thinking of this, Chen Chen, as the investor, replied to the other party with a "pass".
With this, the movie in Hollywood could officially start shooting.
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