Apart from the Sage of the Six Paths, in the original time and space, there are only one and a half pairs of Rinnegan in the Shinobi world.
And this pair and a half of Rinnegan, Sasuke's Rinnegan evolved after the Sage of the Six Paths instilled him with the 'Yin power'. It belongs to the gift of the Sage of the Six Paths. It has no reference value and is extremely difficult to replicate.
Therefore, only Madara Uchiha's method of evolving Rinnegan is really worthy of Hyōjin's research.
Madara Uchiha's Rinnegan is the product of his successful transplantation of the first generation cells after he had the Eternal Mangekyou Sharingan, completing the perfect combination of his own Yin Release and Yang Release.
In other words, according to the example of Madara Uchiha, the Eternal Mangekyou Sharingan representing Yin Release and the first generation cells representing Yang Release are the prerequisites for the evolution of Rinnegan.
But considering that Uchiha is also the reincarnation of Indra, and the first generation Naruto is the reincarnation of Hashirama Asura, they have the Chakra of Indra and Otsutsuki Asura, which is so strong that it can be reincarnated for thousands of years.
So whether the evolution of a pair of Rinnegan requires Indra and Otsutsuki Asura's Chakra becomes a crucial question for Hyōjin to figure out.
Hyōjin's current attempt is to figure out this problem.
He wants to understand the conditions for the evolution of Rinnegan through Zhishui's transplant surgery, whether the Eternal Mangekyou Sharingan perfectly fuses with the first generation cells, or the Chakra of Indra perfectly fuses with Otsutsuki Asura's Chakra.
This is the main reason why he made such a big scene and filled the operating room with all kinds of observation instruments.
If the former was the prerequisite, then Hyōjin was confident that he could cultivate a pair of Reincarnation Eyes.
If the condition is the latter, then it involves the Chakra of Indra and Asura of Otsutsuki who have been reincarnated for thousands of years, then Suijin has to be very careful. After all, the father of these two Specters, the Sage of the Six Paths, is still silently watching the entire shinobi world, and it is hard to say whether he will allow anyone to have any ideas about the Chakra of his two sons, and whether he will allow the third pair of Rinnegan to appear.
Of course, there was another possibility, and that was that these two groups were both necessary conditions to evolve the Eye of Samsara, and neither was dispensable!
After collecting his thoughts, Hyōjin changed into a surgical gown and sterilized all the surgical instruments. Then, he calmly performed the eye transplant surgery on Zhishui.
After implanting the Eternal Mangekyou Sharingan into Zhishui's eye socket, Hyōjin asked, "How do you feel?"
Zhishui took a moment to adapt to the new eye socket, and immediately felt a little puzzled, "I don't know why, but this pair of eyes gives me a very familiar feeling, it's completely different from the first time I transplanted them."
Hyōjin smiled, "That's good."
Uchiha Ryota's Eternal Mangekyou Sharingan has been developed by the Fire Clone, and it has been nourished by the Fire Clone's Chakra for a long time, so it has a high degree of acceptance of the Fire Clone's Chakra.
And Hyōjin's Fire Clone was formed by the fusion of Zhishui's cell tissues, so Zhishui's Chakra is very similar to the Fire Clone's Chakra, so naturally, it is very suitable for this pair of Eternal Mangekyou Sharingan.
After checking the instrument monitoring Zhishui's physical signs, Hyōjin said, "The cell activity in your body has slightly increased, it may be because of the Eternal Mangekyou Sharingan transplant. Try to use your pupil power to see if you can use it normally."
Zhishui closed her eyes, and then suddenly opened them. The original black pupils in her eye sockets have turned into triangular and diamond-shaped Eternal Mangekyou Sharingan.
Hyōjin nodded.
The first step of the surgery has been successfully completed, and the next step is the injection of fusion solution.
The nature of fusion solution is very unstable, and it can only last for an hour after the preparation, it can't be stored for a long time, so after completing the eye exchange, Hyōjin began to prepare the fusion solution on the spot.
Because all the materials and instruments are ready, coupled with Hyōjin's Tenseigan, it didn't take much time to prepare the fusion solution. In less than 30 minutes, a bottle of fusion solution with high activity was successfully prepared by Hyōjin.
Coming to the operating table, Hyōjin said, "Be careful, I'm going to inject the fusion solution."
Zhishui gently nodded, his expression was calm.
Hyōjin poured the fusion solution into the infusion instrument, and soon, the light green fusion solution entered Zhishui's body along the infusion tube.
In an instant, the meridians all over Zhishui's body bulged, as if something fermented in his body, and a layer of sweat appeared on his forehead.
There is no doubt that Zhishui is suffering severe pain at the moment, but he didn't say anything.
At this time, Hyōjin took the first-generation cell tissue that was ready to be implanted into Zhishui's body from the tool table and said, "The effect of the fusion solution is time-limited. I must implant the first-generation cells for you immediately. Are you ready?"
Zhishui gritted his teeth and nodded.
"Don't forget to use the power of the eyes, willpower alone can't resist the erosion of the first-generation cells!"
Hyōjin finally warned, and then officially began the first-generation cell transplantation surgery.
In Hyōjin's view, whether it is Madara Uchiha, Obsidian, or Danzō, their transplantation is not perfect, because they all have obvious traces of transplantation, such as the first-generation face on Madara Uchiha's chest, the upper body of Obsidian, and Danzō's arm that does not belong to him at all.
The real perfect transplantation should be at the genetic level, followed by the cell level, and finally the limb level.
The transplantation of Uchiha Uchiha and Obsidian is between the cell level and the limb level, which is an imperfect cell-level transplantation, and the transplantation of Danzō is obviously at the limb level.
Hyōjin's current transplant surgery on Zhishui is the pursuit of a perfect cell-level transplant.
In short, after the completion of the surgery, Zhishui's physical characteristics will not change, and all the changes in the body will be included at the cell level.
In the entire ninja world, only Hyōjin, Orochi Maru, and Hiruko can reach this level of technology.
"Ah …"
After the first-generation cells were implanted, even a ninja like Zhishui couldn't help but cry out in pain.
"Hold on!"
Hyōjin did not stop and hesitate because of Zhishui's pain. Since the transplant process has begun, there is no turning back, he can only bite the bullet and go all the way.
As more and more first-generation cells were implanted, a lot of granulation emerged on Zhishui's body, like a small sapling that was about to break out of the soil …
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